Nicastrin Antibody

Western Blot: Nicastrin Antibody [NBP2-19538] - Sample (30 ug of whole cell lysate) A: 293T B: HeLa C: HepG2 5% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: Nicastrin Antibody [NBP2-19538] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Product Discontinued

Nicastrin Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Nicastrin. The exact sequence is proprietary.
• Localization: Membrane; Single-pass type I membrane protein (Potential); Melanosome
• Predicted Species: Mouse (92%), Rat (92%), Canine (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCSTN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Nicastrin Antibody

• anterior pharynx-defective 2
• APH2
• ATAG1874
• KIAA0253APH2
• NCSTN
• Nicastrin

Background

This gene encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NCSTN
• Uniprot:
  • Q92542()