Nicastrin Antibody (JG68-33)

Western Blot: Nicastrin Antibody (JG68-33) [NBP2-75592] - Analysis of Nicastrin on THP-1 cell lysate using anti-Nicastrin antibody at 1/1,000 dilution.

Product Discontinued

Nicastrin Antibody (JG68-33) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recomninant protein within Human Nicastrin aa 5-112 / 709. (SwissProt: Q92542 Human)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NCSTN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Nicastrin Antibody (JG68-33)

• anterior pharynx-defective 2
• APH2
• ATAG1874
• KIAA0253APH2
• NCSTN
• Nicastrin

Background

Nicastrin is a large protein that is a component of an enzyme called gamma-secretase, which is lodged in the cell's membrane. Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound gamma-secretase complex. Nicastrin also serves as a docking site for gamma-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to gamma-secretase to ensure the correct cleavage process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCSTN