NFIC Antibody

Western Blot: NFIC Antibody [NBP2-76954] - Western blot analysis of Nfic on different cell lysate using anti-Nfic antibody at 1/500 dilution.Positive control:Lane1: A549Lane2: SiHa

Immunocytochemistry/Immunofluorescence: NFIC Antibody [NBP2-76954] - ICC staining Nfic in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: NFIC Antibody [NBP2-76954] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Nfic antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Flow Cytometry: NFIC Antibody [NBP2-76954] - Flow cytometric analysis of SiHa cells with Nfic antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: NFIC Antibody [NBP2-76954] - ICC staining Nfic in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: NFIC Antibody [NBP2-76954] - ICC staining Nfic in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

NFIC Antibody Summary

• Immunogen: Synthetic peptide within mouse NFIC aa 390-439 / 439. (SwissProt: P08651 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFIC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NFIC Antibody

• CCAAT-binding transcription factor
• CTF5
• CTFCCAAT-box-binding transcription factor
• MGC20153
• NF1-C
• NFI
• NF-I
• NF-I/C
• NFI-C
• nuclear factor 1 C-type
• Nuclear factor 1/C
• nuclear factor I/C (CCAAT-binding transcription factor)
• Nuclear factor I/C
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFIC