Neurogranin Antibody

Western Blot: Neurogranin Antibody [NBP2-29349] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-29349] - Staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Neurogranin Antibody Summary

• Description: Novus Biologicals Goat Neurogranin Antibody (NBP2-29349) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Neurogranin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KIKSGERGRKG, from the internal region of the protein sequence according to NP_006167.1.
• Specificity: Reported variants represent identical protein: NP_006167.1, NP_001119653.1 Immunogen
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRGN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA detection limit 1:8000
  • Western Blot 0.1-0.3ug/ml
• Application Notes: Western blot:Approx 16kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 7.6kDa according to NP_006167.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300; PMID: 9880498).
  IHC: Paraffin embedded Human Brain (Cortex).

Reactivity Notes

Expected from sequence similarity: Mouse, Canine and Bovine. This batch is not expected to cross-react with Mouse in Western blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neurogranin Antibody

• calmodulin-binding protein
• hng
• neurogranin (protein kinase C substrate, RC3)
• Neurogranin
• Ng
• NRGN
• protein kinase C substrate
• RC3
• RC3ng

Background

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurogranin Antibody (NBP2-29349)(1)

Publication using NBP2-29349

• Meneses-San Juan D, Lamas M, Ram�rez-Rodr�guez GB. Repetitive Transcranial Magnetic Stimulation Reduces Depressive-like Behaviors, Modifies Dendritic Plasticity, and Generates Global Epigenetic Changes in the Frontal Cortex and Hippocampus in a Rodent Model of Chronic Stress Cells 2023-08-14 [PMID: 37626872] (Immunohistochemistry)

Bioinformatics

• Gene Symbol: NRGN
• Uniprot:
  • Q92686(Mouse)