Neurogranin Antibody (898502) [Unconjugated]

Western blot shows lysates of human brain (motor cortex), mouse brain, and rat brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Neurogranin Monoclonal Antibody (Catalog # MAB7947) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Neurogranin at approximately 13-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Neurogranin was detected in formalin fixed paraffin-embedded sections of human cortex using Mouse Anti-Human/Mouse/Rat Neurogranin Monoclonal Antibody (Catalog # MAB7947) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and dendrites. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neurogranin was detected in perfusion fixed frozen sections of mouse brain using Mouse Anti-Human/Mouse/Rat Neurogranin Monoclonal Antibody (Catalog # MAB7947) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neurogranin was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat Neurogranin Monoclonal Antibody (Catalog # MAB7947) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 898502
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Neurogranin Antibody (898502) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Neurogranin
  Met1-Asp78
  Accession # Q92686
• Specificity: Detects human Neurogranin in ELISAs.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRGN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurogranin Antibody (898502) [Unconjugated]

• calmodulin-binding protein
• hng
• neurogranin (protein kinase C substrate, RC3)
• Neurogranin
• Ng
• NRGN
• protein kinase C substrate
• RC3
• RC3ng

Background

NRGN (Neurogranin/Ng; also known as RC3, p17 and BICKS) is a member of the neurogranin family of proteins. Although its predicted MW is 7.5 kDa, it runs anomalously at 15-19 kDa in SDS-PAGE. This is apparently due to the adoption of a rigid alpha -helical structure in a negatively charged medium. NRGN has limited expression, being found principally in excitatory neurons of the telencephalon, Golgi and Purkinje cells of the cerebellum, and platelets plus B and T cells in non-nervous tissue. Intracellularly, NRGN is found associated with membranes of the ER, Golgi and mitochondria. This association is often in the form of aggregates (or granules), thus giving rise to its name ("neuro-granules"). NRGN is also found in the nucleus and associated with the postsynaptic spines of dendrites. The principal function of NRGN appears to be the binding, sequestration and concentration of CaM (Calmodulin; a Ca-binding protein) in dendritic spines. Following NMDAR activation, Ca diffuses into the synaptic area, resulting in 1) the simply dissociation of CaM from NRGN, or 2) the phosphorylation of NRGN followed by its dissociation fro CaM. In either case, the freed CaM is now available to activate multiple downstream signaling pathways, some involved in LTP (or memory). Human NRGN is 78 amino acids (aa) in length. It contains one IQ domain (aa 26-47) that binds CaM, and a collagen-like region at the C-terminus (aa 48-78). Regulatory phosphorylation occurs on Ser36, and the N-terminal Met is acetylated. Full-length human NRGN shares 96% aa sequence identity with both mouse and rat NRGN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurogranin Antibody (MAB7947)(2)

Publications using MAB7947

• Kerstin Voelkl; Sara Gutiérrez-Ángel; Sophie Keeling; Seda Koyuncu; Miguel da Silva Padilha; Dennis Feigenbutz et al. Neuroprotective effects of hepatoma-derived growth factor in models of Huntington’s disease Life Science Alliance 2023-11-01 [PMID: 37580082] (Immunohistochemistry, Mouse)
• Dick Jaarsma, Francois G. C. Blot, Bin Wu, Subramanian Venkatesan, Jan Voogd, Dies Meijer et al. The basal interstitial nucleus (BIN) of the cerebellum provides diffuse ascending inhibitory input to the floccular granule cell layer Journal of Comparative Neurology 2018-10-01 [PMID: 29943833]

Bioinformatics

• Gene Symbol: NRGN
• Uniprot:
  • Q92686(Sheep)