Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free

Staining of U-2 OS cells using the Anti-Neurofibromin 1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: CL11865
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free (NBP3-15123) is a monoclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P21359, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free

• DKFZp686J1293
• FLJ21220
• Neurofibromatosis-related protein NF-1
• neurofibromin 1
• neurofibromin
• NFNS
• VRNF
• WSS

Background

Neurofibromin 1 (NF1) is a suspected negative regulator of the ras signal transduction pathway. Neurofibromin is known to regulate the GTPase HRAS, causing the hydrolyzation and inactivation of GTP. Defects in NF1 lead to Neurofibromatosis disease, Recklinghausen disease or Watson disease. Neurofibromin has not been purified, therefore, most of the information regarding this protein has been deduced from homology analysis of its gene sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NF1