NeuroD2 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

NeuroD2 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human NeuroD2
  Ser174-Asn382
  Accession # Q15784
• Specificity: Detects human NeuroD2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human NeuroD1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NeuroD2 Antibody [Alexa Fluor® 700]

• BHLHA1
• bHLHa1neurogenic differentiation factor 2
• Class A basic helix-loop-helix protein 1
• MGC26304
• NDRF
• NDRFneurogenic basic-helix-loop-helix protein
• NeuroD2
• NeuroD-related factor
• neurogenic differentiation 2

Background

NeuroD2 (Neurogenic differentiation factor 2; also NDRF and KW8) is a 41 kDa nuclear member of the neuroD family of transcription factors. It is expressed in developing and mature neurons such as hippocampal granular neurons, and acts (in part) to repress factors that would otherwise block multipotential cell commitment to a neuronal lineage. NeuroD2 is presumed to act as a heterodimer with other bHLH transcription factors. Human NeuroD2 is 382 amino acids (aa) in length. It contains a poly-Glu region (aa 82-91), an NLS (aa 107-113), a DNA-binding HLH domain (aa 119-178) and a poly-Gly segment (aa 282-285). Based on rat studies, human NeuroD2 will undergo variable phosphorylation in the C-terminal region. Over aa 174-382, human and mouse NeuroD2 possess identical amino acid sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.