NETO1 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

NETO1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human NETO1
  Thr23-Val345
  Accession # Q8TDF5
• Specificity: Detects human NETO1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human NETO2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NETO1 Antibody [Alexa Fluor® 350]

• BCTL1
• Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 1
• BTCL1
• BTCL1BCTL1neuropilin and tolloid-like protein 1
• FLJ41325
• NETO1
• neuropilin (NRP) and tolloid (TLL)-like 1

Background

Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina. The 511 amino acid residue mature human NETO1 contains an extracellular domain with two CUB domains (aa 41‑155 and 172‑287), and one class A LDL-receptor segment (aa 292‑326). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation. Over aa 23‑345, human NETO1 shares 97% and 98% aa identity with mouse and canine NETO1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.