NEK3 Antibody

Western Blot: NEK3 Antibody [H00004752-B01P] - Analysis of NEK3 expression in human colon.

Western Blot: NEK3 Antibody [H00004752-B01P] - Analysis of NEK3 expression in A-431.

Western Blot: NEK3 Antibody [H00004752-B01P] - Analysis of NEK3 expression in transfected 293T cell line by NEK3 polyclonal antibody. Lane 1: NEK3 transfected lysate(55.66 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

NEK3 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: NEK3 (NP_002489.1, 1 a.a. - 506 a.a.) full-length human protein. MDDYMVLRMIGEGSFGRALLVQHESSNQMFAMKEIRLPKSFSNTQNSRKEAVLLAKMKHPNIVAFKESFEAEGHLYIVMEYCDGGDLMQKIKQQKGKLFPEDMILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQGCISPLPSHYSYELQFLVKQMFKRNPSHRPSATTLLSRGIVARLVQKCLPPEIIMEYGEEVLEEIKNSKHNTPRKKTNPSRIRIALGNEASTVQEEEQDRKGSHTDLESINENLVESALRRVNREEKGNKSVHLRKASSPNLHRRQWEKNVPNTALTALENASILTSSLTAEDDRGGSVIKYSKNTTRKQWLKETPDTLLNILKNADLSLAFQTYTIYRPGSEGFLKGPLSEETEASDSVDGGHDSVILDPERLEPGLDEEDTDFEEEDDNPDWVSELKKRAGWQGLCDR
• Specificity: NEK3 - NIMA (never in mitosis gene a)-related kinase 3,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: NEK3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEK3 Antibody

• EC 2.7.11
• EC 2.7.11.1
• glycogen synthase A kinase
• HSPK 36
• HSPK36
• hydroxyalkyl-protein kinase
• MGC29949
• Never in mitosis A-related kinase 3
• NIMA (never in mitosis gene a)-related kinase 3
• NimA-related protein kinase 3
• phosphorylase B kinase kinase
• serine/threonine-protein kinase Nek3

Background

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK3
• Entrez:
  • 4752(Human)
• Uniprot:
  • NP_002489.1(Human)