NEDD4 Antibody

Product Discontinued

NEDD4 Antibody Summary

• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the NEDD1 protein.
• Specificity: This antibody reacts with endogenous Nedd4 protein. A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100% homology.
• Predicted Species: Bovine (94%), Rabbit (94%), Primate (94%), Canine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEDD4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000 – 1:35000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:300-1:500
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kDa in size corresponding - Nedd4 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Macaque 100%

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 0.64 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEDD4 Antibody

• Cell proliferation-inducing gene 53 protein
• E3 ubiquitin-protein ligase NEDD4
• EC 6.3.2
• EC 6.3.2.-
• KIAA0093NEDD4-1MGC176705
• NEDD4
• NEDD-4
• Neural precursor cell expressed developmentally down-regulated protein 4
• neural precursor cell expressed, developmentally down-regulated 4
• receptor-potentiating factor 1
• RPF1

Background

NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-beta signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEDD4
• Uniprot:
  • NP_006145()