NEDD4 Antibody (683211) [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 683211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

NEDD4 Antibody (683211) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human NEDD4
  Asn211-Gly349 (predicted)
  Accession # NP_006145
• Specificity: Detects human NEDD4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) NEDD8 or rhNEDD9 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NEDD4 Antibody (683211) [Alexa Fluor® 750]

• Cell proliferation-inducing gene 53 protein
• E3 ubiquitin-protein ligase NEDD4
• EC 6.3.2
• EC 6.3.2.-
• KIAA0093NEDD4-1MGC176705
• NEDD4
• NEDD-4
• Neural precursor cell expressed developmentally down-regulated protein 4
• neural precursor cell expressed, developmentally down-regulated 4
• receptor-potentiating factor 1
• RPF1

Background

NEDD4 (Neural precursor cell-expressed developmentally down-regulated gene 4-1; also RPF1) is a 115-120 kDa member of the HECT E3 ubiquitin ligase family of proteins. The NEDD4 gene is widely expressed, and serves as a complement to a related gene product termed NEDD4-2/NEDD4L. Both molecules ubiquitinate multiple proteins. NEDD4-1 acts preferentially on PTEN, tumor suppressors and endocytic proteins, while NEDD4-2 acts preferentially on membrane transporters. Human NEDD4-1 is 900 amino acids (aa) in length. It contains one PKC region (aa 20-124), four WW domains (aa 196-505) and one E3 HECT domain (aa 543-897). Caspase cleavage after Asp197 generates a 95 kDa C-terminal, and smaller but variable sized N-terminal fragment. There are multiple splice forms. Two show a 516 aa substitution for aa 1-169 and 1-97, respectively, while a third shows the latter substitution coupled to a deletion of aa 170-185. Over aa 211-349, human NEDD4-1 shares 83% aa identity to mouse NEDD4-1 and less than 25% aa identity with human NEDD4-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.