Ndufs4 Antibody (1A1)

Images

 
Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - NDUFS4 monoclonal antibody (M01), clone 1A1 Analysis of NDUFS4 expression in A-431.
Immunohistochemistry-Paraffin: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi ( Cat # H00004724-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody (M01), clone 1A1.Lane 1: NDUFS4 transfected lysate(20.1 KDa).Lane 2: ...read more
Sandwich ELISA: Ndufs4 Antibody (1A1) [H00004724-M01] - Detection limit for recombinant GST tagged NDUFS4 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P, RNAi
Clone
1A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Ndufs4 Antibody (1A1) Summary

Immunogen
NDUFS4 (NP_002486, 66 a.a. - 175 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTADPLSNMVLTFSTKEDAVSFAEKNGWSYDIEERKVPKPKSKSYGANFSWNKRTRVSTK
Specificity
NDUFS4 - NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NDUFS4
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry-Paraffin
  • RNA Inhibition
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ndufs4 Antibody (1A1)

  • AQDQ
  • AQDQmitochondrial
  • CI-18 kDa
  • NADH dehydrogenase (ubiquinone) Fe-S protein 4 (18kD) (NADH-coenzyme Qreductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Qreductase)
  • NADH-coenzyme Q reductase, 18-KD
  • NADH-ubiquinone oxidoreductase 18 kDa subunit

Background

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NDUFS4
Entrez
OMIM
Uniprot