Recombinant Human NDUFB9 His Protein

SDS-Page: NDUFB9 Protein [NBP2-23262]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human NDUFB9 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-179 of Human NDUFB9
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAFLASG PYLTHQQKVL RLYKRALRHL ESWCVQRDKY RYFACLMRAR FEEHKNEKDM AKATQLLKEA EEEFWYRQHP QPYIFPDSPG GTSYERYDCY KVPEWCLDDW HPSEKAMYPD YFAKREQWKK LRRESWEREV KQLQEETPPG GPLTEALPPA RKEGDLPPLW WYIVTRPRER PM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NDUFB9
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 24.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human NDUFB9 His Protein

• B22
• CI-B22
• complex I B22 subunit
• Complex I-B22
• FLJ22885
• LYR motif-containing protein 3
• LYRM3DKFZp566O173
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
• NADH-ubiquinone oxidoreductase B22 subunit
• UQOR22NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (22kD, B22)

Background

NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9, also known as NDUFB9, belongs to the complex I LYR family. Localized to the inner mitochondrial membrane, as well as to the matrix side of the peripheral membrane, NDUFB9 functions as an accessory subunit of the multi-subunit mitochondrial membrane respiratory chain NADH dehydrogenase complex I. Complex I plays an important role in the transfer of electrons from NADH to the respiratory chain, a process that is essential for cellular respiration. Recombinant human NDUFB9 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NDUFB9