Recombinant Human NDUFB4 His Protein

SDS-Page: NDUFB4 Protein [NBP2-23261]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human NDUFB4 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-87 of Human NDUFB4
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSFPKYK PSSLRTLPET LDPAEYNISP ETRRAQAERL AIRAQLKREY LLQYNDPNRR GLIENPALLR WAYARTINVY PNFRPTPKNS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NDUFB4
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 12.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human NDUFB4 His Protein

• B15
• CI-B15
• complex I B15 subunit
• Complex I-B15
• MGC5105
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4 (15kD, B15)
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
• NADH-ubiquinone oxidoreductase B15 subunit

Background

NDUFB4 is a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. Recombinant human NDUFB4 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NDUFB4