NCKAP1 Antibodies
We offer NCKAP1 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each NCKAP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NCKAP1 Antibodies can be used in a variety of model species: Bovine, Canine, Human, Mouse, Porcine, Rat. Use the list below to choose the NCKAP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NCKAP1 polyclonal antibodies.
Alternate Names for NCKAP1 Antibodies
anti-NCKAP1 antibody, anti-FLJ11291 antibody, anti-HEM2p125Nap1 antibody, anti-KIAA0587 antibody, anti-Membrane-associated protein HEM-2 antibody, anti-MGC8981 antibody, anti-NAP 1 antibody, anti-NAP125 antibody, anti-NAP1Nap1 antibody, anti-NCK-associated protein 1 antibody
7 Results for "nckap1" in Primary Antibodies
![Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP2-19491] - NCKAP1 Antibody detects NCKAP1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: NCKAP1 stained by NCKAP1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Immunohistochemistry-Frozen-NBP2-19491-img0003.jpg "Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP2-19491] - NCKAP1 Antibody detects NCKAP1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: NCKAP1 stained by NCKAP1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP2-19491] - Human lung Papillary adenocarcinoma, using NCKAP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-19491-img0002.jpg)
![Western Blot: NCKAP1 Antibody [NBP2-19491] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term (NBP2-19491) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19491_rabbit-polyclonal-nckap1-antibody-91020242014650.jpg)
![Western Blot: NCKAP1 Antibody [NBP2-19491] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term (NBP2-19491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19491_rabbit-polyclonal-nckap1-antibody-910202420115020.jpg)
![Western Blot: NCKAP1 Antibody [NBP2-19491] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term (NBP2-19491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19491_rabbit-polyclonal-nckap1-antibody-9102024195721.jpg)
![Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP1-83269] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody shown in green.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83269-img0006.jpg "Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP1-83269] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269] - Staining in human cerebral cortex and skeletal muscle tissues using anti-NCKAP1 antibody. Corresponding NCKAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83269-img0009.jpg)
![Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83269-img0007.jpg)
![Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83269-img0008.jpg)
![Flow Cytometry: NCKAP1 Antibody [NBP2-26086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-26086_goat-polyclonal-nckap1-antibody-16520239183345.jpg "Flow Cytometry: NCKAP1 Antibody [NBP2-26086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP2-26086] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp2-26086_goat-polyclonal-nckap1-antibody-16520239183343.jpg)
![Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP2-26086] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp2-26086_goat-polyclonal-nckap1-antibody-16520239183318.jpg)
![Western Blot: NCKAP1 Antibody [NBP1-32339] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Western-Blot-NBP1-32339-img0007.jpg "Western Blot: NCKAP1 Antibody [NBP1-32339] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP1-32339] - Frozen-sectioned mouse cerebellum. Green: NCKAP1 stained by NCKAP1 antibody [C1C2], Internal diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Immunohistochemistry-Whole-Mount-NBP1-32339-img0006.jpg)
![Western Blot: NCKAP1 Antibody [NBP1-32339] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Western-Blot-NBP1-32339-img0003.jpg)
![Western Blot: NCKAP1 Antibody [NBP1-32339] - Internal detects NCKAP1 protein by Western blot analysis. A. 50 ug rat brain lysate/extract 5 % SDS-PAGE, dilution: 1:1000](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Western-Blot-NBP1-32339-img0005.jpg)
![Immunoprecipitation:NCKAP1 AntibodyNBP3-29540] -Detection of human NCKAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP1 antibody NBP3-29540 (lot NBP3-29540-1) used for IP at 6 µg per reaction. NCKAP1 was also immunoprecipitated by rabbit anti-NCKAP1.. For blotting immunoprecipitated NCKAP1, NBP3-29540 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29540_rabbit-nckap1-pab-3012202412392115.jpg "Immunoprecipitation:NCKAP1 AntibodyNBP3-29540] -Detection of human NCKAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP1 antibody NBP3-29540 (lot NBP3-29540-1) used for IP at 6 µg per reaction. NCKAP1 was also immunoprecipitated by rabbit anti-NCKAP1.. For blotting immunoprecipitated NCKAP1, NBP3-29540 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: NCKAP1 Antibody [NBP2-93928] - Analysis of extracts of various cell lines, using NCKAP1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/NCKAP1-Antibody-Western-Blot-NBP2-93928-img0001.jpg "Western Blot: NCKAP1 Antibody [NBP2-93928] - Analysis of extracts of various cell lines, using NCKAP1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunoprecipitation:NCKAP1 AntibodyNBP3-29455] -Detection of human NCKAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP1 antibody NBP3-29455 (lot NBP3-29455-1) used for IP at 6 µg per reaction. NCKAP1 was also immunoprecipitated by rabbit anti-NCKAP1.. For blotting immunoprecipitated NCKAP1, NBP3-29455 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29455_rabbit-nckap1-pab-3012202412392137.jpg "Immunoprecipitation:NCKAP1 AntibodyNBP3-29455] -Detection of human NCKAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP1 antibody NBP3-29455 (lot NBP3-29455-1) used for IP at 6 µg per reaction. NCKAP1 was also immunoprecipitated by rabbit anti-NCKAP1.. For blotting immunoprecipitated NCKAP1, NBP3-29455 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")