NCK1 Antibody

Western Blot: NCK1 Antibody [H00004690-D01P] - Analysis of NCK1 expression in transfected 293T cell line by NCK1 polyclonal antibody.Lane 1: NCK1 transfected lysate(42.90 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: NCK1 Antibody [H00004690-D01P] - Analysis of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with anti-NCK1 rabbit purified polyclonal 1:1200 and anti-ABL2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

NCK1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: NCK1 (NP_006144.1, 1 a.a. - 377 a.a.) full-length human protein. MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS
• Specificity: NCK1 - NCK adaptor protein 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NCK1 Antibody

• cytoplasmic protein NCK1
• MGC12668
• NCK adaptor protein 1melanoma NCK protein
• NCK1
• nck-1
• NCKalpha
• NCKNCK tyrosine kinase
• non-catalytic region of tyrosine kinase
• SH2/SH3 adaptor protein NCK-alpha

Background

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCK1
• Entrez:
  • 4690(Human)
• Uniprot:
  • NP_006144.1(Human)