NCK1 Antibody (11D10)

Western Blot: NCK1 Antibody (11D10) [NBP2-11535] - Lane 1: Hela cell lysate. Lane 2: RD cell lysate. Lane 3: PC12 cell lysate. Lane 4: NIH3T3 cell lysate.

Immunohistochemistry-Paraffin: NCK1 Antibody (11D10) [NBP2-11535] - Analysis of anti-NCK1 antibody with human spleen at dilution 1:200.

Immunohistochemistry-Paraffin: NCK1 Antibody (11D10) [NBP2-11535] - Analysis of anti-NCK1 antibody with human tonsil at dilution 1:200.

Product Discontinued

NCK1 Antibody (11D10) Summary

• Immunogen: Recombinant human protein purified from E. coli (His-Nck1).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCK1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: HEPES, 0.01% BSA, 0.15M NaCl and 50% Glycerol
• Preservative: 0.03% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for NCK1 Antibody (11D10)

• cytoplasmic protein NCK1
• MGC12668
• NCK adaptor protein 1melanoma NCK protein
• NCK1
• nck-1
• NCKalpha
• NCKNCK tyrosine kinase
• non-catalytic region of tyrosine kinase
• SH2/SH3 adaptor protein NCK-alpha

Background

Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCK1
• Entrez:
  • 4690(Human)
• Uniprot:
  • P16333(Human)