Nav1.7 Antibody (1D6)

Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody (1D6) [NBP2-75583] - Staining NaV1.7 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Nav1.7 Antibody (1D6) [NBP2-75583] - Analysis of paraffin-embedded human kidney tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin.

Flow Cytometry: Nav1.7 Antibody (1D6) [NBP2-75583] - Analysis of A549 cells with NaV1.7 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody (1D6) [NBP2-75583] - Staining NaV1.7 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Nav1.7 Antibody (1D6) [NBP2-75583] - Analysis of paraffin-embedded human colon cancer tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin.

Product Discontinued

Nav1.7 Antibody (1D6) Summary

• Immunogen: Synthetic peptide within Human Nav1.7 aa 1,577-1,626 / 1,988. (SwissProt: Q15858 Human)
• Localization: Cell membrane. Cell projection.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCN9A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 226 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for Nav1.7 Antibody (1D6)

• ETHA
• FEB3B
• hNE-Na
• Nav1.7
• NENA
• NE-NA
• NENAVoltage-gated sodium channel subunit alpha Nav1.7
• Neuroendocrine sodium channel
• Peripheral sodium channel 1
• PN1
• PN1hNE-Na
• SCN9A
• sodium channel protein type 9 subunit alpha
• Sodium channel protein type IX subunit alpha
• sodium channel, voltage-gated, type IX, alpha subunit
• voltage-gated sodium channel alpha subunit Nav1.7
• voltage-gated, type IX, alpha polypeptide

Background

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (from SwissProt).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCN9A