Recombinant Human NAT1 Protein

SDS-Page: Recombinant Human NAT1 Protein [NBP1-72356] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human NAT1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-290 of Human NAT1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDIEAYLERI GYKKSRNKLD LETLTDILQH QIRAVPFENL NIHCGDAMDL GLEAIFDQVV RRNRGGWCLQ VNHLLYWALT TIGFETTMLG GYVYSTPAKK YSTGMIHLLL QVTIDGRNYI VDAGFGRSYQ MWQPLELISG KDQPQVPCIF RLTEENGFWY LDQIRREQYI PNEEFLHSDL LEDSKYRKIY SFTLKPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPDG VHCLVGFTLT HRRFNYKDNT DLIEFKTLSE EEIEKVLKNI FNISLQRKLV PKHGDRFFTI

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NAT1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Theoretical MW: 36.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human NAT1 Protein

• AAC1N-acetyltransferase type 1
• Arylamide acetylase 1
• arylamine N-acetyltransferase 1
• EC 2.3.1.5
• MNAT
• Monomorphic arylamine N-acetyltransferase
• N-acetyltransferase 1 (arylamine N-acetyltransferase)
• NAT-1
• NATI

Background

NAT1 belongs to the arylamine N-acetyltransferase family. It catalyzes N- or O-acetylation of heterocyclic and arylamine substrates in the detoxification of a wide array of drugs. Certain alleles causing high levels of N-acetyltransferase activity have been associated with colon and urinary bladder cancers, as NAT1 also bioactivate several known carcinogens. This enzyme helps metabolize drugs and other xenobiotics, and participates in the detoxification of a plethora of hydrazine and arylamine drugs. Recombinant human NAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NAT1