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NAT1 Antibody

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ELISA: NAT1 Antibody [24790002]
ELISA: NAT1 Antibody [24790002]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NAT1 Antibody Summary

Immunogen
This antibody is specific for the Middle Region of the target protein.
Specificity
This product is specific for Human NAT1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:2000
Application Notes
This antibody is useful in ELISA, Western Blot and Immunohistochemistry-Paraffin.
Publications
Read Publications using 24790002.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for NAT1 Antibody

  • AAC1N-acetyltransferase type 1
  • Arylamide acetylase 1
  • arylamine N-acetyltransferase 1
  • EC 2.3.1.5
  • MNAT
  • Monomorphic arylamine N-acetyltransferase
  • N-acetyltransferase 1 (arylamine N-acetyltransferase)
  • NAT-1
  • NATI

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: NAT2 Antibody [NBP1-33751] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: NAT2 Antibody [NBP1-33751] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
Species: Hu
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SLC38A1 Antibody
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Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32. Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody at 1:1000.
Immunocytochemistry/Immunofluorescence: SLC38A1 Antibody (S104-32) [NBP2-59311] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SNAT1 Antibody (D) Composite.
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NARG1L Antibody
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Immunocytochemistry/Immunofluorescence: NARG1L Antibody [NBP1-92167] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: NARG1L Antibody [NBP1-92167] - Staining of human appendix shows moderate cytoplasmic positivity in glandular cells.
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BRD2 Antibody
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Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 used at a dilution of 1:100 (2 ug/mL) (left) and rabbit anti-BRD2 recombinant monoclonal used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.
Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-30475] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (NBP1-30475). Detection: DAB
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Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).
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NBP1-32733
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Species: Hu, Mu, Rt
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Cytochrome P450
1A1/1A2 Antibody (MC1
NB100-74398
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Mouse liver tissue (right) compared with a negative control in the absence of primary antibody (left).
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1A2 Antibody (OTI8F1)
NBP2-02563
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Glutathione
S-Transferase pi 1/GS ...
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(1E10)
H00002946-M03
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Cytosolic
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Immunocytochemistry/Immunofluorescence: SULT1A1 Antibody (9B7) [NBP2-03227] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SULT1A1.
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Cytochrome P450
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NBP1-85367
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Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human endometrium shows no positivity in glandular or stromal cells as expected.
Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Analysis in human liver tissue.
(1 Publication)
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Adenine Nucleotide
Translocator 2 Antibo ...
NBP2-57642
Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells and cells in granular layer.
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Western Blot: EPHX1 Antibody [NBP2-38594] - Analysis using Anti-EPHX1 antibody NBP2-38594 (A) shows similar pattern to independent antibody NBP1-83969 (B).
Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP2-38594] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP2-38594] - Staining of human tonsil shows low expression as expected.
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Glutathione S
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NBP2-80461
Western Blot: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Western blot analysis of GSTK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: SK-BR-3 cell lysateLane 2: Human skin tissue lysateLane 3: Human placenta tissue lysate
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GSTK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Flow cytometric analysis of GSTK1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1:100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Publications for NAT1 Antibody (24790002)(2)

We have publications tested in 1 application: MiAr.


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Bioinformatics

Gene Symbol NAT1
Entrez
Uniprot