Recombinant NARS Protein

Product Discontinued

Recombinant NARS Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 568 amino acids corresponding to Brungia malawi Asparagine tRNA ligase.
  
  

  Source: Escherichia coli.

  Amino Acid Sequence:
  MGSSHHHHHH SSGLVPRGSH MTVYICPETG DDGNDGSELK PLRTLYQAMI ITKSSKGDFL IRTKKDGKQV WEAASKTALK KSWKRYEQEM LKNEKVAAKM LEKDATEVGV KAALEEAKKV QIELDTSLSY ITGVKIRDLV KHRNERVCIK GWIHRMRRQG KSLMFFILRD GTGFLQVLLM DKLCQTYDAL TVNTECTVEI YGAIKEVPEG KEAPNGHELI ADFWKIIGNA PSGGIDNVLN EEASVDKMLD NRHLVIRGEN AAALLRLRAA ATRAMREHFY NAGYVEVAPP TLVQTQVEGG STLFNLDYFG EQSFLTQSSQ LYLETCIPTL GDVFLHCSVL QGGKISHSST LAEYAHVEAE CPFITLDDLM EKIEELVCDT VDRLLADEEA KKLLEHINPK FQPPERPFLR MEYKDAIKWL QEHNVENEFG NTFTYGEDIA EAAERFMTDT INKPILLNRF PSEIKAFYMQ RDAKDNTLTE SVDLLMPGVG EIVGGSMRIW KFDELSKAFK NVEIDPKPYY WYLDQRLYGT CPHGGYGLGL ERFICWLTNT NHIRDVCLYP RFVGRCVP

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NARS1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Less than 1 EU/ug of NARS as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Functional
• Theoretical MW: 64.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in 20 mM HEPES, pH 7.4, 100 mM NaCl, 5 mM MgCl2, 5 mM beta-Mercaptoethanol, 10 % Glycerol
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant NARS Protein

• ASNRS
• asparagine tRNA ligase 1, cytoplasmic
• Asparagine--tRNA ligase
• asparaginyl-tRNA synthetase
• asparaginyl-tRNA synthetase, cytoplasmic
• EC 6.1.1.22
• NARS1

Background

The Bungia malayi asparagine-tRNA ligase (also known as asparaginyl-tRNA synthetase/AsnRs) catalyses the chemical reaction: ATP + L-asparagine + tRNAAsn to AMP + diphosphate + L-asparaginyl-tRNAAsn. AsnRS is an alpha2 dimer that belongs to class IIb, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. AsnRS is considered an excellent antifilarial target to cure for Lymphatic filariasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NARS1