Human NAPA ELISA Kit (Colorimetric)

ELISA: Human NAPA ELISA Kit (Colorimetric) [NBP2-60604] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 1.875 - 60 ng/mL (example only; lot dependent)
• Sensitivity: 0.15 ng/mL (example only; lot dependent)

Human NAPA ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 1.875 - 60 ng/mL (example only; lot dependent)
• Sensitivity: 0.15 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10.0% (example only; lot dependent)
• Intra-Assay: %CV < 5.5% (example only; lot dependent)
• Spike Recovery: 89 - 113% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NAPA

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human NAPA ELISA Kit (Colorimetric)

• alpha-SNAP
• alpha-soluble NSF attachment protein
• N-ethylmaleimide-sensitive factor attachment protein alpha
• N-ethylmaleimide-sensitive factor attachment protein, alpha
• S
• SNAPA
• SNAP-alpha

Background

The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for NAPA ELISA Kit (NBP2-60604). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NAPA