NAPA Antibody (15D4)

Product Discontinued

NAPA Antibody (15D4) Summary

• Immunogen: Balb/C mice were immunized with recombinant human -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by western blot. The antibody was purified to >95% homogeneity by standard chromatographic techniques
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAPA
• Purity: Chromatography

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, and 50% glycerol
• Preservative: No Preservative
• Purity: Chromatography

Alternate Names for NAPA Antibody (15D4)

• alpha-SNAP
• alpha-soluble NSF attachment protein
• N-ethylmaleimide-sensitive factor attachment protein alpha
• N-ethylmaleimide-sensitive factor attachment protein, alpha
• S
• SNAPA
• SNAP-alpha

Background

SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicleto-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta -SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAPA