myosin X Antibody

Images

 
Western Blot: myosin X Antibody [22430002] - Detection of Myo10 in HeLa cells at a dilution of 1:800 with 1 mg/mL stock. The predicted molecular weight of MYO10, based on the amino acid sequence, is 237 kD. MYO10 ...read more
Immunocytochemistry/ Immunofluorescence: myosin X Antibody [22430002] - The effect of HOXA5 on single cell migration. The median speed was calculated by tracking the movement of individual cells with around 25 cells per ...read more
Immunohistochemistry: myosin X Antibody [22430002] - Most of the normal tissues showed moderate cytoplasmic positivity. Strong staining was observed in stomach, rectum, exocrine pancreas, urothelium and in placenta. ...read more
Immunocytochemistry/ Immunofluorescence: myosin X Antibody [22430002] - staining of Actin at a dilution of 1:800. Image courtesy of MM DiVito and RE Cheney, Department of Cell and Molecular Physiology, University of ...read more
Immunocytochemistry/ Immunofluorescence: myosin X Antibody [22430002] - Myosins are actin-based motor molecules with ATPase activity. MYO10 plays a role in regions of dynamic actin and is presumed to serve in ...read more
Immunocytochemistry/ Immunofluorescence: myosin X Antibody [22430002] - staining of Myo10 at a dilution of 1:800 with 1.0mg/mL stock overlapped with immunofluorescence staining of Actin at a dilution of 1:800. Image ...read more
ELISA: myosin X Antibody [22430002]

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

myosin X Antibody Summary

Immunogen
This antibody is specific for the Middle Region of the target protein (NM_012334).
Specificity
This product is specific for Human Myosin 10.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYO10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:800
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This antibody is useful in ELISA, Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot.
Publications
Read Publications using
22430002 in the following applications:

Reactivity Notes

Human. mouse reactivity reported in scientific literature (PMID: 24270889)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for myosin X Antibody

  • FLJ10639
  • FLJ21066
  • FLJ22268
  • KIAA0799FLJ43256
  • MGC131988
  • myosin X
  • myosin-X
  • Unconventional myosin-10

Background

Myosin motor domain. This catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Myosins are actin-dependent molecular motors that play important roles in muscle contraction, cell motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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22430002
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Supplier Logo

Publications for myosin X Antibody (22430002)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, MiAr, WB.


Filter By Application
ICC/IF
(5)
IHC
(1)
IHC-P
(1)
MiAr
(2)
WB
(5)
All Applications
Filter By Species
Human
(7)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 22430002 Applications Species
Lehtimaki JI, Fenix AM, Kotila TM et al. UNC-45a promotes myosin folding and stress fiber assembly. J. Cell Biol. 2017 Oct 20 [PMID: 29055011] (WB) WB
Jacquemet G, Baghirov H, Georgiadou M et al. L-type calcium channels regulate filopodia stability and cancer cell invasion downstream of integrin signalling. Nat Commun. Dec 2 2016 [PMID: 27910855] (WB, Human) WB Human
Margiotta A, Progida C, Bakke O, Bucci C. Rab7a regulates cell migration through Rac1 and vimentin. Biochim Biophys Acta 2017 Feb [PMID: 27888097] (Human) Human
Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation and Pathway Interconnections with Obesity. Journal of Proteome Research Oct 21 2016 [PMID: 27769113] (MiAr)

Details:
myosin X Antibody used in microarray creation
MiAr
Osswald M, Jung E, Sahm F et al. Brain tumour cells interconnect to a functional and resistant network. Nature. 2015 Dec 03 [PMID: 26536111] (ICC/IF) ICC/IF
Wang CC, Su KY, Chen HY et al. HOXA5 Inhibits Metastasis via Regulating Cytoskeletal Remodelling and Associates with Prolonged Survival in Non-Small-Cell Lung Carcinoma PLoS ONE 2015 Apr 16 [PMID: 25875824] (ICC/IF, Human) ICC/IF Human
Wang Y, Brooks PJ, Jang JJ et al. Role of actin filaments in fusopod formation and osteoclastogenesis Biochim. Biophys. Acta 2015 Apr 12 [PMID: 25871908] (ICC/IF, Mouse) ICC/IF Mouse
Arjonen A, Kaukonen R, Mattila E et al. Mutant p53-associated myosin-X upregulation promotes breast cancer invasion and metastasis. J. Clin. Invest. 2014 Feb 3 [PMID: 24487586] (WB, IHC-P, Human, Mouse) WB, IHC-P Human, Mouse
Fierro-Gonzalez JC, White MD, Silva JC, Plachta N. Cadherin-dependent filopodia control preimplantation embryo compaction. Nat Cell Biol. 2013 Dec [PMID: 24270889] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays. Journal of Proteome Research May 3 2014 [PMID: 23541305] (MiAr)

Details:
Anti-myosin X used in screening array
MiAr
Show All 13 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for myosin X Antibody (22430002). (Showing 1 - 1 of 1 FAQs).

  1. Can you tell me the antigen sequence of Myosin 10 Antibody (22430002).
    • This is a product that there is not additional immunogen sequence information available for, as it is deemed proprietary.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MYO10
Entrez
Uniprot