Myosin light chain kinase Antibody

ELISA: Myosin light chain kinase Antibody [32180002]

Product Discontinued

Myosin light chain kinase Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein.
• Specificity: This product is specific for Human MYLK.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYLK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This product is useful in Immunohistochemistry-Paraffin and has been QC tested in ELISA against recombinant protein only.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Myosin light chain kinase Antibody

• DKFZp686I10125
• EC 2.7.11
• EC 2.7.11.18
• FLJ12216
• Kinase-related protein
• KRP
• light polypeptide kinase
• MLCK1
• MLCK108
• MLCKMLCK210
• MSTP083
• MYLK1
• myosin light chain kinase
• smooth muscle myosin light chain kinase
• smooth muscle
• telokin

Background

Myosin light chain kinase (MLCK) is a serine/threonine kinase that belongs to CaM Kinases (Ca++/Calmodulin-dependent protein kinases) family. MLCK is involved in the regulation of myosin activity during smooth muscle contraction. At increased level of Ca2+, rapid binding of Ca2+ to Calmodulin (CaM) occurs, triggering MLCK activation. Once activated, MLCK phosphorylates myosin light chain (MLC) and leading to cross-bridges cycling and generation of the contractile force (1). MLCK has been implicated in the regulation of endothelial and vascular permeability and in signaling pathway which leads to fibroblast apoptosis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin light chain kinase Antibody (32180002)(2)

Publications using 32180002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: MYLK
• Entrez:
  • 4638(Human)
• Uniprot:
  • Q15746(Human)