MxA/Mx1 Antibody

Western Blot: MxA/Mx1 Antibody [H00004599-D01P] - Analysis of MX1 expression in human kidney.

Immunocytochemistry/Immunofluorescence: MxA/Mx1 Antibody [H00004599-D01P] - Analysis of antibody to MX1 on HeLa cell. Antibody concentration 30 ug/ml.

Western Blot: MxA/Mx1 Antibody [H00004599-D01P] - Analysis of MX1 expression in transfected 293T cell line by MX1 polyclonal antibody.Lane 1: MX1 transfected lysate(75.50 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

MxA/Mx1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MX1 (NP_002453.1, 1 a.a. - 662 a.a.) full-length human protein. MVVSEVDIAKADPAAASHPLLLNGDATVAQKNPGSVAENNLCSQYEEKVRPCIDLIDSLRALGVEQDLALPAIAVIGDQSSGKSSVLEALSGVALPRGSGIVTRCPLVLKLKKLVNEDKWRGKVSYQDYEIEISDASEVEKEINKAQNAIAGEGMGISHELITLEISSRDVPDLTLIDLPGITRVAVGNQPADIGYKIKTLIKKYIQRQETISLVVVPSNVDIATTEALSMAQEVDPEGDRTIGILTKPDLVDKGTEDKVVDVVRNLVFHLKKGYMIVKCRGQQEIQDQLSLSEALQREKIFFENHPYFRDLLEEGKATVPCLAEKLTSELITHICKSLPLLENQIKETHQRITEELQKYGVDIPEDENEKMFFLIDKINAFNQDITALMQGEETVGEEDIRLFTRLRHEFHKWSTIIENNFQEGHKILSRKIQKFENQYRGRELPGFVNYRTFETIVKQQIKALEEPAVDMLHTVTDMVRLAFTDVSIKNFEEFFNLHRTAKSKIEDIRAEQEREGEKLIRLHFQMEQIVYCQDQVYRGALQKVREKELEEEKKKKSWDFGAFQSSSATDSSMEEIFQHLMAYHQEASKRISSHIPLIIQFFMLQTYGQQLQKAMLQLLQDKDTYSWLLKERSDTSDKRKFLKERLARLTQARRRLAQFPG
• Specificity: Reacts with myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • SDS-Page
  • Western Blot
• Application Notes: Antibody reactivity against tissue and transfected lysate in WB, and as a detection antibody in ELISA. SDS-Page usage reported in scientific literature (PMID: 26507657).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MxA/Mx1 Antibody

• human interferon-regulated resistance GTP-binding protein MXA10IFI-78Khomolog of murine (interferon-inducibleprotein p78)
• IFI78
• IFI-78K
• Mx1
• MxA
• myxovirus (influenza virus) resistance 1, interferon-inducible protein p78(mouse)

Background

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MxA/Mx1 Antibody (H00004599-D01P)(3)

Publications using H00004599-D01P

• Nigg PE, Pavlovic J. Oligomerization and GTP-binding requirements of MxA for viral target recognition and antiviral activity against influenza A virus. J. Biol. Chem. 2015-10-27 [PMID: 26507657] (WB, PAGE, Human)
• Moschonas G, Delhaye L, Cooreman R et al. MX2 restricts HIV-1 and herpes simplex virus-1 by forming cytoplasmic biomolecular condensates that mimic nuclear pore complexes bioRxiv 2023-06-24 (WB, Human)
  
  Details:
  Dilution 1:5000
• Crameri M, Bauer M, Caduff N et al. MxB is an interferon-induced restriction factor of human herpesviruses Nat Commun 2018-05-17 [PMID: 29773792] (WB, Human)

Bioinformatics

• Gene Symbol: MX1
• Entrez:
  • 4599(Human)
• Uniprot:
  • NP_002453.1(Human)