Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody

Product Discontinued

Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 311-360 of Human mAChR M1.
• Specificity: mAChR M1 (R336) detects endogenous levels of mAChR M1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHRM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of mAChR M1 (R336) pAb in extracts from hela cells.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody

• cholinergic receptor, muscarinic 1
• CHRM1
• HM1
• M1
• M1R
• mAChR M1
• MGC30125
• muscarinic acetylcholine receptor M1

Background

The muscarinic acetylcholine receptors (mAChR) mediate a variety of cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels. The mAChRs transducer signals by coupling to G-proteins, which then modulate several downstream effector proteins and ion channels. Five mAChR subtypes have been identified, designated M1 to M5. The five receptor subtypes show distinct patterns of tissue distribution, as well as distinct pharmacological and functional properties. The amino acid sequence of each mAChR subtype reflects a structure that is characteristic of G-protein coupled receptors, consisting of seven highly conserved transmembrane segments and a large intracellular region unique to each subtype, which constitutes the effector-coupling domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody (NBP1-00905). (Showing 1 - 1 of 1 FAQ).

1. Can I use this antibody (mAChR1) for immunohistochemistry (brain slice)?
   • Our muscarinic acetylcholine receptor 1 antibody with catalogue number NBP1-00905 has been validated for Western blotting with samples from human, mouse and rat, however we have not tested it in any other applications.

Bioinformatics

• Gene Symbol: CHRM1
• Entrez:
  • 1128(Human)