Musashi-2 Antibody (ST59-02)

Western Blot: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Analysis of MSI2 on different lysates using anti-MSI2 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo Lane 2: PC-12

Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Analysis of paraffin-embedded mouse prostate tissue using anti-MSI2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Analysis of Hela cells with MSI2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Staining MSI2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Staining MSI2 in Lovo cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Analysis of paraffin-embedded human liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Analysis of paraffin-embedded human pancreas tissue using anti-MSI2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Analysis of paraffin-embedded mouse heart tissue using anti-MSI2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Musashi-2 Antibody (ST59-02) [NBP2-67547] - Analysis of paraffin-embedded mouse liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.

Product Discontinued

Musashi-2 Antibody (ST59-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Musashi-2 aa 180-300 / 328. (SwissProt: Q96DH6 Huma; SwissProt: Q920Q6 Mouse; Entrez Gene: 360596 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MSI2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Musashi-2 Antibody (ST59-02)

• FLJ36569
• MGC3245
• MSI2
• MSI2H
• musashi homolog 2 (Drosophila)
• Musashi2
• Musashi-2
• RNA-binding protein Musashi homolog 2

Background

Musashi 2 encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSI2