Mus81 Antibody (MTA30 2G10/3) - BSA Free

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: MTA30 2G10/3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Mus81 Antibody (MTA30 2G10/3) - BSA Free Summary

• Immunogen: Human MUS81
• Localization: Nuclear
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUS81
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: MTA30 2G10/3 is recommended for WB of recombinant protein, human whole cell extracts, native IP and fractionated cell extracts at a concentration of 1:1000. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Mus81 Antibody (MTA30 2G10/3) - BSA Free

• crossover junction endonuclease MUS81
• EC 3.1.22
• EC 3.1.22.-
• FLJ21012
• FLJ44872
• MUS81 endonuclease homolog (S. cerevisiae)
• MUS81 endonuclease homolog (yeast)
• SLX3 structure-specific endonuclease subunit homolog
• SLX3

Background

Mus81 and EME1 constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. Reduction or loss of a protein called Mus81 can led to the development of lymphomas and other tumours in mice. Mus81 is a protein long thought to be involved in repairing DNA that is damaged during normal metabolic processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mus81 Antibody (NB100-2064)(2)

Publications using NB100-2064

• Benitez A, Yuan F, Nakajima S et al. Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein. Nucleic Acids Res. 2013-10-28 [PMID: 24170812] (WB, Human)
• Rodrigue A, Coulombe Y, Jacquet K et al. The RAD51 paralogs ensure cellular protection against mitotic defects and aneuploidy. J Cell Sci. 2013-01-01 [PMID: 23108668] (WB, Human)

Bioinformatics

• Gene Symbol: MUS81
• Entrez:
  • 80198(Human)