mu Opioid R/OPRM1 Antibody

Immunohistochemistry-Frozen: mu Opioid R/OPRM1 Antibody [NB100-1620] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.

Immunocytochemistry/Immunofluorescence: mu Opioid R/OPRM1 Antibody [NB100-1620] - Labeling showing the presence of a subpopulation of spinal lamina II neurons that contained both u opioid receptor (green) and TRPV1 (red) immunoreactivity in a vehicle (A)- and a RTX (B)-treated rats. Scale bar, 20 um. More information found: J Neurophysiol 95: 3086-3096, 2006. First published February 8, 2006; doi:10.1152/jn.01343.2005 0022-3077

Immunocytochemistry/Immunofluorescence: mu Opioid R/OPMR1 Antibody [NB100-1620] - Staining of Rat DRG.

Immunohistochemistry: mu Opioid R/OPMR1 Antibody [NB100-1620] - Striatum

Product Discontinued

mu Opioid R/OPRM1 Antibody Summary

• Immunogen: Synthetic peptide: NHQLENLEAETAPLP, corresponding to C terminal amino acids 384-398 of Rat Mu Opioid Receptor.
• Localization: Integral membrane protein.
• Specificity: Mu Opioid Receptor
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OPRM1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:2000
  • Immunohistochemistry 1:100 - 1:2000
  • Immunohistochemistry-Frozen
  • Western Blot 1:500 - 1:1000
• Application Notes: Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32807797).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for mu Opioid R/OPRM1 Antibody

• hMOP
• KIAA0403
• LMOR
• MOP
• mOpioidR
• MOR
• MOR1
• M-OR-1
• MOR-1
• MOR1MOR
• Mu opiate receptor
• mu Opioid R
• Mu opioid receptor
• mu-type opioid receptor
• opioid receptor, mu 1
• OPRM
• OPRM1

Background

The powerful effects of opiate are mediated through the opioid receptors located on the plasma membrane of neurons and other peripheral cells. There are at least 3 types of opioid receptors, mu, kappa, and delta, each with a distinct pharmacologic profile. The mu opioid receptor is the primary site of action for the most commonly used opioids, including morphine, heroin, fentanyl, and methadone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mu Opioid R/OPRM1 Antibody (NB100-1620)(5)

Publications using NB100-1620

• Ronstr�m JW, Johnson NL, Jones ST et al. Opioid-Induced Reductions in Amygdala Lateral Paracapsular GABA Neuron Circuit Activity International Journal of Molecular Sciences 2023-01-18 [PMID: 36768252] (Western Blot, Rat)
• Zhao Y, Ren X, Li F et al. P2Y1 receptor in the colonic submucosa of rats and its association with opioid induced constipation Experimental and Therapeutic Medicine 2022-12-13 [PMID: 36605532] (WB, Rat)
• Subramaniyam S, Yang S, Diallo BN et al. Oral Phyto-thymol ameliorates the stress induced IBS symptoms Sci Rep 2020-08-17 [PMID: 32807797] (IHC-Fr, Rat)
• Jiao X, Wang X, Wang R et al. Rules to activate CD8+T cells through regulating subunits of opioid receptors by methionine enkephalin (MENk). Int. Immunopharmacol. 2018-10-02 [PMID: 30290369] (WB, Mouse)
• Vassoler FM, Wright SJ, Byrnes EM. Exposure to opiates in female adolescents alters mu opiate receptor expression and increases the rewarding effects of morphine in future offspring. Neuropharmacology. 2015-12-15 [PMID: 26700246] (WB, Rat)
  
  Details:
  mu Opioid R/OPMR1 antibody was used in WB assays for detecting the Mu Opioid Receptor /MOR expression in the Nac / nucleus accumbens (Fig. 5, Panel A) and VTA/ventral tegmental area of P60 rats (Fig. 5, Panel B).

Bioinformatics

• Gene Symbol: OPRM1
• Entrez:
  • 4988(Human)
  • 18390(Mouse)
  • 25601(Rat)
• Uniprot:
  • P33535()