Recombinant Human mu Crystallin His Protein

SDS-Page: mu Crystallin Protein [NBP1-44383] - CRYM, 35.9 kDa (334aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human mu Crystallin His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-314 of Human mu Crystallin
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSRVPAFLSA AEVEEHLRSS SLLIPPLETA LANFSSGPEG GVMQPVRTVV PVTKHRGYLG VMPAYSAAED ALTTKLVTFY EDRGITSVVP SHQATVLLFE PSNGTLLAVM DGNVITAKRT AAVSAIATKF LKPPSSEVLC ILGAGVQAYS HYEIFTEQFS FKEVRIWNRT KENAEKFADT VQGEVRVCSS VQEAVAGADV IITVTLATEP ILFGEWVKPG AHINAVGASR PDWRELDDEL MKEAVLYVDS QEAALKESGD VLLSGAEIFA ELGEVIKGVK PAHCEKTTVF KSLGMAVEDT VAAKLIYDSW SSGK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CRYM
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 35.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human mu Crystallin His Protein

• crystallin, mu
• DFNA40NADP-regulated thyroid-hormone binding protein
• mu-crystallin homolog
• NADP-regulated thyroid-hormone-binding protein
• THBP

Background

Crystallin mu, also known as CRYM, is a member of the crystallin protein family. Crystallins are separated into two classes, taxon-specific and ubiquitous. This gene encodes a taxon-specific crystallin protein. The human CRYM gene maps to chromosome 16p13.11, and encodes a protein that is expressed in neural tissue, muscle, and kidney. Unlike other crystallins, CRYM does not perform a structural role in lens tissue, but rather it binds NADPH and thyroid hormone, which indicates that it may have other regulatory or developmental functions. Recombinant human CRYM, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CRYM