MTAP Antibody

Images

 
Western Blot: MTAP Antibody [NBP1-32718] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody diluted at a dilution of 1:2000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: MTAP Antibody [NBP1-32718] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP antibody [N1C3] diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: MTAP Antibody [NBP1-32718] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.
Western Blot: MTAP Antibody [NBP1-32718] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:1000.
Immunoprecipitation: MTAP Antibody [NBP1-32718] - Immunoprecipitation of MTAP protein from HeLa whole cell extracts using 5 ug of MTAP antibody [N1C3]. Western blot analysis was performed using MTAP antibody [N1C3]. ...read more

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MTAP Antibody Summary

Immunogen
Full length human MTAP Recombinant protein.
Localization
Cytoplasm
Predicted Species
Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (80%), Chicken (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for MTAP Antibody

  • 5'-methylthioadenosine phosphorylase
  • c86fus
  • EC 2.4.2.28
  • methylthioadenosine phosphorylase
  • MSAPMeSAdo phosphorylase
  • MTA phosphorylase
  • MTAPase
  • S-methyl-5'-thioadenosine phosphorylase

Background

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MTAP Antibody (NBP1-32718) (0)

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Secondary Antibodies

 

Isotype Controls

Additional MTAP Products

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Diseases for MTAP Antibody (NBP1-32718)

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Calculators

Concentration Calculator

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Bioinformatics

Gene Symbol MTAP