MSP/MST1 Antibody [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

MSP/MST1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse MSP/MST1
  Gly19-Gly716 (Cys677Ala)
  Accession # NP_032269
• Specificity: Detects mouse MSP/MST1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human MSP is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSP/MST1 Antibody [Alexa Fluor® 594]

• D3F15S2
• EC 3.4.21
• hepatocyte growth factor-like protein homolog
• HGFL
• HGFLhepatocyte growth factor-like protein
• macrophage stimulating 1 (hepatocyte growth factor-like)
• Macrophage stimulatory protein
• Macrophage-stimulating protein
• MSP
• MSPDNF15S2
• MST1
• NF15S2
• SF2

Background

MSP (Macrophage stimulating protein 1; also hepatocyte growth factor-like protein) is an 80-95 kDa member of the peptidase S1 family of proteins. Although it is expressed principally by hepatocytes, it can also be induced in cells such as renal tubular epithelium. MSP has multiple targets, and as such, has multiple effects. It stimulates macrophage motility and phagocytosis, promotes keratinocyte and renal tubular cell proliferation, and depresses myeloid progenitor cell replication. Mouse proMSP is 698 amino acids (aa) in length. It contains one PAN (carbohydrate-binding) site (aa 19-105), four consecutive kringle domains (110-457) and an inactive peptidase S1 region (aa 489-714). An intrachain disulfide bond exists between Cys477 and Cys593 that becomes an interchain bond following protease cleavage between Arg488 and Val489. This creates a mature heterodimer containing a 45-57 kDa alpha -chain, and a 30-35 kDa beta -chain. Mouse proMSP shares 80% and 93% aa identity with human and rat proMSP, respectively.

                        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.