MSP/MST1 Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 647

MSP/MST1 Antibody [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human MSP/MST1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human HGF is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSP/MST1 Antibody [Alexa Fluor™ Plus 647]

• D3F15S2
• EC 3.4.21
• hepatocyte growth factor-like protein homolog
• HGFL
• HGFLhepatocyte growth factor-like protein
• macrophage stimulating 1 (hepatocyte growth factor-like)
• Macrophage stimulatory protein
• Macrophage-stimulating protein
• MSP
• MSPDNF15S2
• MST1
• NF15S2
• SF2

Background

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45‑62 kDa alpha and a 25‑35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12).

1. Wang, M.-H. et al. (2002) Scand. J. Immunol. 56:545.
2. Han, S. et al. (1991) Biochemistry 30:9768.
3. Muraoka, R.S. et al. (1999) Endocrinology 140:187.
4. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
5. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
6. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
7. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
8. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
9. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
10. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
11. Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
12. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.