MSK2 Antibody (1013D) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 1013D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

MSK2 Antibody (1013D) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human MSK1(S376) site.
  Accession # O75582
• Specificity: Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSK2 Antibody (1013D) [Alexa Fluor® 532]

• MSK2
• ribosomal protein S6 kinase, 90kDa, polypeptide 4
• RPS6KA4
• RSKB

Background

Mitogen- and Stress-activated protein Kinases 1 and 2 (MSK1/2) have been shown to play key roles in the transcriptional regulation of immediate early genes such as c-fos. MSK1, also known as Ribosomal Protein S6 Kinase 5 (RPS6KA5), and MSK2, also known as RSKB and RPS6KA4, belong to the AGC family of kinases. Both proteins have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB. The phosphorylation of MSK1 at Ser376 or the equivalent Ser360 in MSK2 is required for kinase activity. These sites are located in the AGC kinase domain and are autophosphorylated. Their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. The sequence surrounding MSK1(S376) and MSK2(S360) is 100% identical. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.