MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05)

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05) [NBP2-67653] - Staining MSK1(phospho S376) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05) [NBP2-67653] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MSK1/RPS6KA5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05) [NBP2-67653] - Staining MSK1(phospho S376) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05) [NBP2-67653] - Staining MSK1(phospho S376) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05) [NBP2-67653] - Staining MSK1(phospho S376) in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05) [NBP2-67653] - Analysis of paraffin-embedded mouse bladder tissue using anti-MSK1(phospho S376) antibody. Counter stained with hematoxylin.

Product Discontinued

MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser376 of Human MSK1/RPS6KA5 aa 351-400 / 802. (SwissProt: O75582 Human; Unigene:8940 Rat)
• Modification: p Ser376
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPS6KA5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MSK1/RPS6KA5 [p Ser376] Antibody (SN63-05)

• EC 2.7.11
• MGC1911,90 kDa ribosomal protein S6 kinase 5
• MSK1
• MSK1EC 2.7.11.1
• MSPK1
• Nuclear mitogen- and stress-activated protein kinase 1
• ribosomal protein S6 kinase alpha-5
• ribosomal protein S6 kinase, 90kD, polypeptide 5
• ribosomal protein S6 kinase, 90kDa, polypeptide 5
• RLPK
• RPS6KA5
• RSKL
• RSK-like protein kinase
• S6K-alpha-5

Background

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA5