MRPS34 Antibody (OTI4H2)

Western Blot: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Human Kidney tissue.

Western Blot: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS34.

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Human colon tissue.

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Adenocarcinoma of Human colon tissue.

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Human bladder tissue.

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Human pancreas tissue.

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Carcinoma of Human thyroid tissue.

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of Human tonsil tissue.

Immunofluorescence: MRPS34 Antibody (OTI4H2) [NBP2-45432] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MRPS34.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI4H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MRPS34 Antibody (OTI4H2) Summary

• Immunogen: Full length human recombinant protein of human MRPS34(NP_076425) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRPS34
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 25.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MRPS34 Antibody (OTI4H2)

• MGC2616
• mitochondrial ribosomal protein S34
• MRPS12
• MRP-S12,28S ribosomal protein S34, mitochondrial
• MRP-S34
• S34mt

Background

MRPS34, also known as Mitochondrial Ribosomal Protein S34, consists of a 218 amino acid isoform that is 26 kDa, and is a portion of the 28S small mitochondrial ribosome subunit, which is involved in protein synthesis. MRPS34 is not currently being utilized in any disease research. The protein interacts with ICT1, MYC, NFKBIL1, USP42, and DLG1. MRPS34 is not linked to any pathways or biological processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRPS34 Antibody (NBP2-45432)(1)

Publication using NBP2-45432

• Lavie J, Lalou C, Mahfouf W et al. The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins Cell reports 2023-05-31 [PMID: 37267103] (WB, Human)

Bioinformatics

• Gene Symbol: MRPS34