MRPL49 Antibody

Western Blot: MRPL49 Antibody [NBP3-06296] - Anti-MRPL49 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: RT4 Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:17 kDa

Immunoprecipitation: MRPL49 Antibody [NBP3-06296] - MRPL49 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-MRPL49 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-MRPL49 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size: 17 kDa

Product Discontinued

MRPL49 Antibody Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human MRPL49 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL49
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH7.0
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MRPL49 Antibody

• C11orf4
• L49mt39S ribosomal protein L49, mitochondrial
• mitochondrial ribosomal protein L49
• MRP-L49
• Neighbor of FAU
• next to FAU
• NOF1chromosome 11 open reading frame 4
• NOFMGC10656
• Protein NOF1

Background

MRPL49, or Mitochondrial Ribosomal Protein L49, consists of a 166 amino acid isoform that is 19 kDa, and is broadly associated with mitochondrial protein synthesis, though its specific function is still being researched. Current disease research is determining the relationship between MRPL49 and a variety of diseases and disorders, including glossopharyngeal neuralgia, osteoarthritis, ossifying fibroma, osteoporosis, and thoracic outlet syndrome. The protein has been linked to translation, and interacts with ICT1, OXA1L, SlC2A4, AARS2, and AASS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL49