MRLC2 Overexpression Lysate (Native)

Images

 
Western Blot: MRLC2 Overexpression Lysate (Adult Normal) [NBL1-13229] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MRLC2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MRLC2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201372 and based on accession number NM_033546. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens myosin, light chain 12B, regulatory (MYL12B), transcript variant 2, mRNA.
Gene
MYL12B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MRLC2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MRLC2 Overexpression Lysate (Native)

  • MLC-2
  • MLC20
  • MLC-2A
  • MLC-B
  • MRLC2SHUJUN-1
  • MYLC2B
  • myosin regulatory light chain 12B
  • myosin regulatory light chain 2
  • Myosin regulatory light chain 20 kDa
  • Myosin regulatory light chain 2-B, smooth muscle isoform
  • Myosin regulatory light chain MRLC2
  • myosin, light chain 12B, regulatory

Background

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same isoform (A).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30249
Species: Hu, Mu, Rt, Xp
Applications: WB, ELISA, Flow, ICC/IF
NBP1-87744
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-86648
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-80073
Species: Hu, Rt
Applications: WB, IHC, IHC-P
H00085366-M01
Species: Hu
Applications: WB, ELISA, RNAi
NBP2-01462
Species: Hu
Applications: WB, Flow, ICC/IF
NBP1-47602
Species: Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
NB100-91273
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-14871
Species: Hu, Mu
Applications: WB, ICC/IF
NB120-2860
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, ICC, IF
NBP1-98474
Species: Hu
Applications: WB
NB110-38889
Species: Hu
Applications: WB, IP
NBP1-02417
Species: Hu, Mu, Rt, Av, Ma
Applications: WB, ELISA, IHC, IHC-P, IP
NBP2-14873
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-37897
Species: Hu
Applications: WB, IHC, IHC-P
NB100-624
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBL1-13229
Species: Hu
Applications: WB

Publications for MRLC2 Lysate (NBL1-13229) (0)

There are no publications for MRLC2 Lysate (NBL1-13229).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MRLC2 Lysate (NBL1-13229) (0)

There are no reviews for MRLC2 Lysate (NBL1-13229). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MRLC2 Lysate (NBL1-13229) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MRLC2 Products

Bioinformatics Tool for MRLC2 Lysate (NBL1-13229)

Discover related pathways, diseases and genes to MRLC2 Lysate (NBL1-13229). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MRLC2 Lysate (NBL1-13229)

Discover more about diseases related to MRLC2 Lysate (NBL1-13229).
 

Pathways for MRLC2 Lysate (NBL1-13229)

View related products by pathway.

PTMs for MRLC2 Lysate (NBL1-13229)

Learn more about PTMs related to MRLC2 Lysate (NBL1-13229).

Blogs on MRLC2

There are no specific blogs for MRLC2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

MRLC2 Antibody
NBP2-54719
ROCK1 Antibody
NB100-624

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MRLC2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol MYL12B