MRGX2 Antibody

Immunohistochemistry-Paraffin: MRGX2 Antibody [NBP1-02669] - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry-Paraffin: MRGX2 Antibody [NBP1-02669] - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Product Discontinued

MRGX2 Antibody Summary

• Immunogen: Synthetic 16 amino acid peptide from C-terminal cytoplasmic domain of human MRGX2.
• Epitope: C-Terminus cytoplasmic domain
• Specificity: Human MRGX2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Orangutan (94%), Chimpanzee (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRGPRX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 10-20 ug/ml
• Application Notes: IHC: NBP1-02669 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (88%), Monkey (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MRGX2 Antibody

• MAS-related GPR, member X2
• mas-related G-protein coupled receptor member X2
• MRGPRX2
• MRGX2
• MRGX2G protein-coupled receptor MRGX2

Background

MRGX2 is highly similar to other MRG receptors. Recently it has been shown that MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRGPRX2
• Entrez:
  • 117194(Human)
• Uniprot:
  • Q96LB1()