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MPP3 Antibody Pair

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Sandwich ELISA: MPP3 Antibody Pair [H00004356-AP11] - Detection sensitivity ranging from 1 ng/ml to 100 ng/ml.
Sandwich ELISA: MPP3 Antibody Pair [H00004356-AP11] - Detection sensitivity ranging from 1 ng/ml to 100 ng/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications S-ELISA
Clonality
Monoclonal
Standard Curve Range
1 ng/ml to 100 ng/ml

Order Details

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MPP3 Antibody Pair Summary

Description
This antibody pair set comes with matched antibody pair to detect and quantify protein level of human MPP3.

  • Capture antibody: rabbit MaxPab affinity purified polyclonal anti-MPP3 (100 ug)
  • Detection antibody: mouse monoclonal anti-MPP3 IgG1 Kappa (20 ug)
    		•
    Standard Curve Range
    1 ng/ml to 100 ng/ml
    Assay Type
    Sandwich ELISA
    Clonality
    Monoclonal
    Gene
    MPP3

    Applications/Dilutions

    Dilutions
    • Sandwich ELISA 1 ng/ml - 100 ng/ml

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative

    Notes

    Quality control test: Standard curve using recombinant protein (H00004356-P01) as an analyte.

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for MPP3 Antibody Pair

    • Discs large homolog 3
    • discs, large homolog 3
    • DLG3MAGUK p55 subfamily member 3
    • membrane protein palmitoylated 3
    • membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
    • Protein MPP3

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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    NB600-1229
    Western Blot: DLG1 Antibody [NB600-1229] - Analysis of 25 ug of C6 (lane 1), PC12 (lane 2) and rat brain (lane 3) cell lysates.
    Immunocytochemistry/Immunofluorescence: DLG1 Antibody [NB600-1229] - Analysis of SAP97 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SAP97 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
    Immunocytochemistry/Immunofluorescence: DLG1 Antibody [NB600-1229] - Analysis of SAP97 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SAP97 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
    (1 Publication    )
    Species: Hu, Mu, Rt
    Applications: WB, ICC/IF, IHC, IHC-Fr, IP
    Guanylate kinase
    Antibody (OTI4A8)
    NBP2-00594
    Western Blot: Guanylate kinase Antibody (4A8) [NBP2-00594] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Guanylate kinase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Guanylate kinase.
    Immunohistochemistry-Paraffin: Guanylate kinase Antibody (4A8) [NBP2-00594] Staining of paraffin-embedded Human prostate tissue using anti-Guanylate kinase mouse monoclonal antibody.
    Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Guanylate kinase antibody, and then analyzed by flow cytometry.
    Species: Hu
    Applications: WB, Flow, IHC, IHC-P

    IGSF4A/SynCAM1/CADM1 A
    NB300-186
    Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY CADM1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SynCAM/CADM1.
    Immunocytochemistry/Immunofluorescence: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - SynCAM/CADM1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
    Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.
    (1 Publication    )
    Species: Hu, Rt
    Applications: WB, Simple Western, ICC/IF
    PSD93 Antibody
    NBP1-88088
    Immunocytochemistry/Immunofluorescence: PSD93 Antibody [NBP1-88088] - Staining of human cell line A-431 shows positivity in nucleus, nucleoli and vesicles.
    Immunohistochemistry-Paraffin: PSD93 Antibody [NBP1-88088] - Staining of human duodenum shows low expression as expected.
    Immunohistochemistry-Paraffin: PSD93 Antibody [NBP1-88088] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
    Species: Hu
    Applications: ICC/IF, IHC, IHC-P
    MPP2 Antibody
    NBP1-85038
    Western Blot: MPP2 Antibody [NBP1-85038] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
    Immunocytochemistry/Immunofluorescence: MPP2 Antibody [NBP1-85038] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
    Immunohistochemistry-Paraffin: MPP2 Antibody [NBP1-85038] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
    Species: Hu
    Applications: WB, ICC/IF, IHC, IHC-P
    PSD-95 Antibody
    (6G6-1C9)
    NB300-556
    Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.
    Immunocytochemistry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected. This image was submitted via customer Review.
    Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
    (1 Review    )
    (12 Publications    )
    Species: Hu, Mu, Rt, In, Pm
    Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC
    PSD-95 Antibody
    (6G6-1C9)
    NB300-556
    Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.
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    Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
    (1 Review    )
    (12 Publications    )
    Species: Hu, Mu, Rt, In, Pm
    Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC
    EPB41L3 Antibody
    NB110-61027
    Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in mouse brain lysate
    Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in HeLa whole cell lysate
    Simple Western: EPB41L3 Antibody [NB110-61027] - Simple Western lane view shows a specific band for EPB41L3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
    (2 Publications    )
    Species: Hu, Mu, Rt, Pm, Xp
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    MPP1 Antibody
    (1E11-1G11)
    H00004354-M01
    Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - MPP1 monoclonal antibody (M01), clone 1E11-1G11. Analysis of MPP1 expression in human placenta.
    Immunocytochemistry/Immunofluorescence: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of monoclonal antibody to MPP1 on HeLa cell. Antibody concentration 10 ug/ml.
    Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.
    Species: Hu
    Applications: WB, ELISA, ICC/IF, IP
    Lgl1 Antibody (5G2)
    H00003996-M01
    Western Blot: Lgl1 Antibody (5G2) [H00003996-M01] - Western Blot analysis of LLGL1 expression in Hela NE ( Cat # L013V3 ).
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    (3 Publications    )
    Species: Hu
    Applications: WB, ELISA
    FoxM1 Antibody
    NBP1-30961
    Western Blot: FoxM1 Antibody [NBP1-30961] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term diluted at 1:5000.
    Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-30961] - Paraffin-embedded OVCA, using antibody at 1:100 dilution.
    (2 Publications    )
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    Fascin Antibody
    (833223) [Unconjugate
    MAB7745
    Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fascin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
    Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Fascin at approximately 61 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
    Fascin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
    (2 Reviews    )
    Species: Hu
    Applications: WB, Simple Western, IHC
    NDOR1 Antibody
    NBP1-88790
    Western Blot: NDOR1 Antibody [NBP1-88790] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
    Immunocytochemistry/Immunofluorescence: NDOR1 Antibody [NBP1-88790] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & intermediate filaments.
    Immunohistochemistry-Paraffin: NDOR1 Antibody [NBP1-88790] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
    Species: Hu, Mu, Rt
    Applications: WB, ICC/IF, IHC, IHC-P
    ERG Antibody
    NBP2-60655
    Western Blot: ERG Antibody [NBP2-60655] - Total protein from Human THP-1, Jurkat and K562 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ERG in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
    Immunocytochemistry/Immunofluorescence: ERG Antibody [NBP2-60655] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ERG at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
    Immunohistochemistry-Paraffin: ERG Antibody [NBP2-60655] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney (NBP1-78269) using 1:100 dilution of ERG antibody. The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a nuclear staining with weak cytoplasmic positivity in the cells of the glomeruli and the tubular epithelial cells.
    Species: Hu
    Applications: WB, ICC/IF, IHC, IHC-P
    Protein Disulfide
    Isomerase/P4HB Antibo ...
    H00005034-B01P
    Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in human liver.
    Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of purified antibody to P4HB on HeLa cell. (antibody concentration 10 ug/ml)
    Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in HeLa.
    (1 Review    )
    (2 Publications    )
    Species: Hu
    Applications: WB, ICC/IF
    VAM1 Antibody
    NB100-1366
    Western Blot: VAM1 Antibody [NB100-1366] - Western blot analysis of PALS2 / VAM1 / MPP6 in 293 lysate using NB100-1366 at 0.5 ug/ ml.
    Western Blot: VAM1 Antibody [NB100-1366] - (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    (1 Publication    )
    Species: Hu
    Applications: WB, PEP-ELISA
    POLG2 Antibody
    NBP1-83227
    Western Blot: POLG2 Antibody [NBP1-83227] - Analysis in human cell line RH-30.
    Immunohistochemistry-Paraffin: POLG2 Antibody [NBP1-83227] - Staining of human testis shows positivity in Leydig cells.
    Immunohistochemistry-Paraffin: POLG2 Antibody [NBP1-83227] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.
    Species: Hu, Mu(-)
    Applications: WB, IHC, IHC-P
    MPP3 Antibody Pair
    H00004356-AP11
    MPP3 Antibody Pair
    Species: Hu
    Applications: S-ELISA

    Publications for MPP3 Antibody Pair (H00004356-AP11) (0)

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    Bioinformatics

    Gene Symbol MPP3