Mouse IFN-alpha All Subtype Quantikine ELISA Kit

IFN-alpha samples containing a high concentration in various matrices are diluted with appropriate Calibrator Diluent to produce sample values within the dynamic range of the assay. R&D Systems natural linearity is between 98%-103% compared to 125%-180% for the top competitor.

IFN-alpha is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 88%-92% compared to 132%-201% for the top competitor.

IFN-alpha is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 99%.

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IFN-alpha All Subtype Quantikine ELISA Kit Summary

• Background: The Quantikine™ Mouse IFN-alpha All Subtype immunoassay is a 3.5 hour solid-phase ELISA designed to measure IFN-alpha in mouse cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant mouse IFN-alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IFN-alpha showed dose response curves that were paralle...l to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-alpha .
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• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IFN-alpha All Subtype Quantikine ELISA Kit

• IFNA
• IFNA2
• IFNA2b
• IFNalpha
• IFN-alpha
• IFN-alphaA
• INFA2
• interferon alpha 2
• LeIF D

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Publications for IFN-alpha (MFNAS0)(1)

Publication using MFNAS0

• Wang, J;Jin, X;Yan, S;Zhao, H;Pang, D;Ouyang, H;Tang, X; Yeast ?-glucan promotes antiviral type I interferon response via dectin-1 Veterinary microbiology 2024-05-09 [PMID: 38838382] (Mouse)

FAQs for IFN-alpha (MFNAS0). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.