MNK1 Overexpression Lysate

Western Blot: MNK1 Overexpression Lysate (Adult Normal) [NBL1-13123] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MNK1.

• Reactivity: Hu
• Applications: WB

MNK1 Overexpression Lysate Summary

• Description:

  MNK1 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC201149
  
  Accession#: NM_003684
  
  Protein Tag: C-MYC/DDK
• Gene: MKNK1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 51.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MNK1 Overexpression Lysate

• EC 2.7.11
• EC 2.7.11.1
• MAP kinase interacting kinase 1
• MAP kinase interacting serine/threonine kinase 1
• MAP kinase signal-integrating kinase 1
• MAP kinase-interacting serine/threonine-protein kinase 1
• MKNK1
• MNK1
• MNK1MAP kinase-interacting serine/threonine kinase 1

Background

MNK1 may play a role in the response to environmental stress and cytokines. It appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MKNK1