Recombinant Human MMP-13 Protein

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SDS-Page: Recombinant Human MMP-13 Protein [NBP1-99622]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, HPLC
Concentration
0.15 mg/ml

Order Details

Recombinant Human MMP-13 Protein Summary

Description
A recombinant protein corresponding to Human MMP-13
Source
Sf 9 (baculovirus)
Protein/Peptide Type
Biologically Active Protein
Gene
MMP13
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Application Notes
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30C. Assay conditions: 25 mM HEPES pH 7.5 2 mM MgCl2 1.0 mM DTT 0.5 mM NADP 2 mM ATP 25 mM glucose 100ug/ml BSA 20 units/ml glucose 6-phosphate dehydrogenase and 10 nM human liver glucokinase at 30C for 30 min.
Theoretical MW
53.82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris pH 6.5, 250 mM NaCl, 5 mM CaCl2, 1 mM ZnCl2
Concentration
0.15 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human MMP-13 Protein

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

MMP-13 (Collagenase-3) was first identified in human mammacarcinoma (Freije et al., 1994, Willmroth et al. 1998) - probably induced by IL1- alpha and IL-1 beta - and shown to be glycosylated and the inactive zymogen displaying a relative molecular weight of 60 kDa. Cleavage of the 84 residue propeptide can be catalysed by other MMPs such as MMP-2, MMP-3 and MMP-14, or by factors like plasmin. The proenzyme activated by APMA (paminohenylmercuric acteate) or leads to the active enzyme with a relative molecular weight of app. 48 kDa which easily autodegrades into a 30 kDa form. This highly active 30 kDa form still retains the characteristics of the app. 48 kDa form. MMP-13 also plays a central role in the MMP activation cascade, both activating and being activated by several MMPs (Leeman et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol MMP13
Entrez