Human MMP-13 ELISA Kit (Colorimetric)

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ELISA: Human MMP-13 ELISA Kit (Colorimetric) [KA1600] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1600
    • Availability
      Product Discontinued

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Human MMP-13 ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard curve. MMP13 (Human) ELISA Kit provides a highly sensitive and specific quantitative determination of activated human MMP13.
Standard Curve Range
32 to 2000 pg/mL
Sensitivity
7 pg/mL
Assay Type
Quantification
Inter-Assay
CV% < 4.8%
Intra-Assay
CV% < 5.875%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MMP13

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Assay Buffer: Phosphate buffer with additives.
  2. Biotinylated Antibody: Solution contains biotinylated monoclonal anti-procollagenase 3antibody in buffer with additives. The antibody has to be diluted 100-fold with DetectionBuffer before use.
  3. Conjugate Solution: This solution contains a highly polymeric streptavidin-peroxidaseconjugate with preservatives. It has to be diluted 40-fold with Detection Buffer prior touse.
  4. Detection Buffer: Solution consists of phosphate buffer containing additives.
  5. Microtiter plate: The plate contains 6 x 16 strips coated with anti-MMP-13.
  6. Serum Standard Diluent: This solution contains certified human serum in a buffer withadditives. Ready to use. Warning: Component contains human serum! Wear eye, hand,face, and clothing protection when using this material!
  7. Standard: Lyophilized act-MMP-13 in Assay Buffer. It has to be reconstituted with 1 ml ofdistilled water prior to use to get a 20 ng/mL stock solution.
  8. Stop Solution: 0.25 M H2SO4. Ready to use. Warning: Stop Solution contains 0.25 Msulphuric acid. Wear eye, hand, face, and clothing protection when using this material!
  9. TMB Substrate: ready to use.
  10. Wash Buffer Concentrate

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human MMP-13 ELISA Kit (Colorimetric)

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for MMP-13 ELISA Kit (KA1600). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol MMP13