Human MMP-13 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. MMP13 (Human) ELISA Kit provides a highly sensitive and specific quantitative determination of activated human MMP13. |
| Standard Curve Range |
32 to 2000 pg/mL |
| Sensitivity |
7 pg/mL |
| Assay Type |
Quantification |
| Inter-Assay |
CV% < 4.8% |
| Intra-Assay |
CV% < 5.875% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
MMP13 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Assay Buffer: Phosphate buffer with additives.
- Biotinylated Antibody: Solution contains biotinylated monoclonal anti-procollagenase 3antibody in buffer with additives. The antibody has to be diluted 100-fold with DetectionBuffer before use.
- Conjugate Solution: This solution contains a highly polymeric streptavidin-peroxidaseconjugate with preservatives. It has to be diluted 40-fold with Detection Buffer prior touse.
- Detection Buffer: Solution consists of phosphate buffer containing additives.
- Microtiter plate: The plate contains 6 x 16 strips coated with anti-MMP-13.
- Serum Standard Diluent: This solution contains certified human serum in a buffer withadditives. Ready to use. Warning: Component contains human serum! Wear eye, hand,face, and clothing protection when using this material!
- Standard: Lyophilized act-MMP-13 in Assay Buffer. It has to be reconstituted with 1 ml ofdistilled water prior to use to get a 20 ng/mL stock solution.
- Stop Solution: 0.25 M H2SO4. Ready to use. Warning: Stop Solution contains 0.25 Msulphuric acid. Wear eye, hand, face, and clothing protection when using this material!
- TMB Substrate: ready to use.
- Wash Buffer Concentrate
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human MMP-13 ELISA Kit (Colorimetric)
Background
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for MMP-13 ELISA Kit (KA1600). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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