Human MMP-13 ELISA Kit (Colorimetric) Summary
Description |
Quality control test: Standard curve. MMP13 (Human) ELISA Kit provides a highly sensitive and specific quantitative determination of activated human MMP13. |
Standard Curve Range |
32 to 2000 pg/mL |
Sensitivity |
7 pg/mL |
Assay Type |
Quantification |
Inter-Assay |
CV% < 4.8% |
Intra-Assay |
CV% < 5.875% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
MMP13 |
Applications/Dilutions
Dilutions |
|
Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
Components
|
- Assay Buffer: Phosphate buffer with additives.
- Biotinylated Antibody: Solution contains biotinylated monoclonal anti-procollagenase 3antibody in buffer with additives. The antibody has to be diluted 100-fold with DetectionBuffer before use.
- Conjugate Solution: This solution contains a highly polymeric streptavidin-peroxidaseconjugate with preservatives. It has to be diluted 40-fold with Detection Buffer prior touse.
- Detection Buffer: Solution consists of phosphate buffer containing additives.
- Microtiter plate: The plate contains 6 x 16 strips coated with anti-MMP-13.
- Serum Standard Diluent: This solution contains certified human serum in a buffer withadditives. Ready to use. Warning: Component contains human serum! Wear eye, hand,face, and clothing protection when using this material!
- Standard: Lyophilized act-MMP-13 in Assay Buffer. It has to be reconstituted with 1 ml ofdistilled water prior to use to get a 20 ng/mL stock solution.
- Stop Solution: 0.25 M H2SO4. Ready to use. Warning: Stop Solution contains 0.25 Msulphuric acid. Wear eye, hand, face, and clothing protection when using this material!
- TMB Substrate: ready to use.
- Wash Buffer Concentrate
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human MMP-13 ELISA Kit (Colorimetric)
Background
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for MMP-13 ELISA Kit (KA1600). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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