MLK3 Antibody

Western Blot: MLK3 Antibody [NBP1-45711] - Analysis of MLK3 expression with HeLa cell lysate.

Product Discontinued

MLK3 Antibody Summary

• Immunogen: Synthetic peptide mapping to the C-terminus of human MLK3.
• Specificity: It does not cross-react with MLK1, MLK2 and other mixed lineage kinases.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K11
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 50% Glycerol
• Preservative: 0.02% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for MLK3 Antibody

• EC 2.7.11
• EC 2.7.11.25
• MAP3K11
• MEKK11
• mitogen-activated protein kinase kinase kinase 11
• Mixed lineage kinase 3
• MLK3
• MLK3MLK-3
• PTK1
• PTK1protein-tyrosine kinase PTK1
• RHOE
• SH3 domain-containing proline-rich kinase
• SPRK
• SPRKMGC17114
• Src-homology 3 domain-containing proline-rich kinase

Background

Mixed lineage kinase-3 (MLK-3) is a mitogen-activated kinase kinase kinase that mediates stress-activating protein kinase (SAPK)/c-Jun NH2-terminal kinase activation. MLK-3 and other MLK family kinases are characterized by the presence of multiple protein-protein interaction domains including a tandem leucine/isoleucine zipper (LZs) motif. Leucine zippers are known to mediate protein dimerization raising the possibility that the tandem leucine/isoleucine zippers may function as a dimerization motif of MLK-3 (1). MLK-3 has several interesting structural features including an SH3 domain in the absence of an SH2 domain, a region containing two leucine zippers with an adjacent carboxy-terminal basic region, and a proline rich region. (2). HPK1 has been found to phosphorylate MLK-3 activation loop in vitro, and Ser281 was found to be the major phosphorylation site, indicating that HPK1 also activates MLK-3 via phosphorylation of the kinase activation loop (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MLK3 Antibody (NBP1-45711)(1)

Publication using NBP1-45711

• Chien ST, Lin SS, Wang CK et al. Acacetin inhibits the invasion and migration of human non-small cell lung cancer A549 cells by suppressing the p38a MAPK signaling pathway Mol Cell Biochem 2011-04-01 [PMID: 21210297] (WB, Human)

Bioinformatics

• Gene Symbol: MAP3K11
• Entrez:
  • 4296(Human)
  • 26403(Mouse)
  • 309168(Rat)
• Uniprot:
  • Q16584()