MKP-3/DUSP6 Antibody

Western Blot: MKP-3/DUSP6 Antibody [NBP2-26174] - Human (A), (Mouse) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: MKP-3/DUSP6 Antibody [NBP2-26174] - Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MKP-3/DUSP6 Antibody Summary

• Immunogen: Peptide with sequence C-PSNQNVYQVDSLQST corresponding to C-Terminus according to NP_073143.2.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001937.2; NP_073143.2).
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DUSP6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in mouse and rat heart lysates and 40 kDa band plus 26 kDa band bands observed in human and porcine heart lysates (calculated MW of 42.3 kDa band according to NP_001937.2 and 26.5 kDa band according to NP_073143.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MKP-3/DUSP6 Antibody

• dual specificity phosphatase 6
• Dual specificity protein phosphatase PYST1
• DUSP6
• EC 3.1.3.16
• EC 3.1.3.48
• MAP kinase phosphatase 3
• Mitogen-activated protein kinase phosphatase 3
• MKP3
• MKP-3
• MKP3serine/threonine specific protein phosphatase
• PYST1
• PYST1dual specificity protein phosphatase 6

Background

DUSP6 is encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKP-3/DUSP6 Antibody (NBP2-26174)(1)

Publication using NBP2-26174

• Geetha N, Mihaly J, Stockenhuber A et al. Signal integration and coincidence detection in the mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) cascade: concomitant activation of receptor tyrosine kinases and of LRP-1 leads to sustained ERK phosphorylation via down-regulation of dual specificity phosphatases (DUSP1 and -6). J Biol Chem 2011-07-22 [PMID: 21610072]

Bioinformatics

• Gene Symbol: DUSP6
• Entrez:
  • 1848(Human)
  • 67603(Mouse)
  • 116663(Rat)