MKP-3/DUSP6 Antibody (SR39-09)

Western Blot: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Analysis of DUSP6 on mouse pancreas lysates using anti-DUSP6 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Staining DUSP6 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-MKP-3/DUSP6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked

Flow Cytometry: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Analysis of NIH/3T3 cells with DUSP6 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Staining DUSP6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Staining DUSP6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Analysis of paraffin-embedded human pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Analysis of paraffin-embedded mouse brain tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (SR39-09) [NBP2-67320] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.

Product Discontinued

MKP-3/DUSP6 Antibody (SR39-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human MKP-3/DUSP6 aa 325-381 / 381. (SwissProt: Q16828 Human; SwissProt: Q9DBB1 Mouse; SwissProt: Q64346 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DUSP6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MKP-3/DUSP6 Antibody (SR39-09)

• dual specificity phosphatase 6
• Dual specificity protein phosphatase PYST1
• DUSP6
• EC 3.1.3.16
• EC 3.1.3.48
• MAP kinase phosphatase 3
• Mitogen-activated protein kinase phosphatase 3
• MKP3
• MKP-3
• MKP3serine/threonine specific protein phosphatase
• PYST1
• PYST1dual specificity protein phosphatase 6

Background

DUSP6 is encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKP-3/DUSP6 Antibody (NBP2-67320)(1)

Publication using NBP2-67320

• James NE, Beffa L, Oliver MT et al. Inhibition of DUSP6 sensitizes ovarian cancer cells to chemotherapeutic agents via regulation of ERK signaling response genes Oncotarget 2019-05-21 [PMID: 31164954] (WB, Human)

Bioinformatics

• Gene Symbol: DUSP6