MKP-1/DUSP1 Antibody

Images

 
Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Staining of HeLa cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Rt, Mu, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MKP-1/DUSP1 Antibody Summary

Immunogen
Peptide with sequence SYLQSPITTSPSC corresponding to C-Terminus according to NP_004408.1.
Predicted Species
Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DUSP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 8 ug/mL
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 40 kDa band observed in human lysates of HeLa (calculated MW of 39.3 kDa band according to NP_004408.1). Customers have reported positive results on mouse microglia (BV2 cells), rat primary microglia, and rat spinal cord homogenate.
Reviewed Applications
Read 1 Review rated 4
using
NB100-834 in the following applications:

Publications
Read Publication using NB100-834.

Reactivity Notes

Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MKP-1/DUSP1 Antibody

  • CL100
  • CL100MAP kinase phosphatase 1
  • dual specificity phosphatase 1
  • Dual specificity protein phosphatase hVH1
  • DUSP1
  • EC 3.1.3.16
  • EC 3.1.3.48
  • HVH1
  • Mitogen-activated protein kinase phosphatase 1
  • MKP1
  • MKP-1
  • MKP-1dual specificity protein phosphatase 1
  • Protein-tyrosine phosphatase CL100
  • PTPN10
  • PTPN10serine/threonine specific protein phosphatase
  • VH1

Background

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MKP-1/DUSP1 Antibody (NB100-834)(1)

Review for MKP-1/DUSP1 Antibody (NB100-834) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-834:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot MKP-1/DUSP1 NB100-834
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4
reviewed by:
Alexander Vang
WB Rat 03/19/2018
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Summary

ApplicationWestern Blot
Sample TestedHeart lysates
SpeciesRat
LotS2 E190417

Comments

CommentsProteins were separated on SDS-PAGE and transferred onto PVDF membrane. Membrane was blocked with 5% milk in TBS 0.01% tween and then incubated with primary antibody overnight 1:1000. Secondary was used at 1:5000 and then membrane was ECL and imaged with GE Imagequant imager.

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Video Protocols

WB Video Protocol

FAQs for MKP-1/DUSP1 Antibody (NB100-834) (0)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Alexander Vang
03/19/2018
Application: WB
Species: Rat

Bioinformatics

Gene Symbol DUSP1
Entrez
Uniprot