MKLP1 Antibody (SY02-74)

Western Blot: MKLP1 Antibody (SY02-74) [NBP2-67446] - Western blot analysis of MKLP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: MKLP1 Antibody (SY02-74) [NBP2-67446] - Staining MKLP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: MKLP1 Antibody (SY02-74) [NBP2-67446] - Analysis of MKLP1 on different lysates using anti-MKLP1 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela

Immunocytochemistry/Immunofluorescence: MKLP1 Antibody (SY02-74) [NBP2-67446] - Staining MKLP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

MKLP1 Antibody (SY02-74) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MKLP1 aa 801-850 / 960. (SwissProt: Q02241 Human; Unigene: 259374 Mouse; Unigene: 63734 Rat)
• Localization: Cytoplasm, Nucleus, Midbody.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KIF23
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MKLP1 Antibody (SY02-74)

• kinesin family member 23
• kinesin-like 5 (mitotic kinesin-like protein 1)
• Kinesin-like protein 5
• kinesin-like protein KIF23
• KNSL5CHO1
• mitotic kinesin-like 1
• MKLP-1
• MKLP1Mitotic kinesin-like protein 1

Background

Using the CHO1 monoclonal antibody, Sellitto and Kuriyama (1988) identified a spindle antigen that was shown by Nislow et al. (1990) to be required for mitotic progression. By using the CHO1 antibody to screen a HeLa cell cDNA library, Nislow et al. (1992) isolated a KNSL5 cDNA, which they called MKLP1 (mitotic kinesin-like protein-1), that encodes this antigen. The N-terminal region of the deduced 961-amino acid protein contains a 350-residue domain that shares 30 to 45% identity with the motor domains of other members of the kinesin superfamily. The C-terminal region shows little identity with other kinesins. A central region contains heptad repeats of hydrophobic residues that may assemble into a coiled-coil structure similar to kinesin and myosin heavy chain proteins. The protein also contains a putative nuclear localization signal and 2 consensus phosphorylation sites characteristic of nuclear proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIF23